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Results 1 - 9 of 9
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16A264G relative hydrolytic activity: 20.4% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16D54N inactive mutant, used for the structural analysis of the complex with sucrose 729994
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16more residues that are critical to the intramolecular fructosyl transfer reaction of LFTase are analysed. An error-prone PCR mutagenesis process is carried out, and the mutants that led to a shift in activity from transfructosylation towards hydrolysis of levan are screened by the dinitrosalicylic acid (DNS) method. Selected mutants reveal two major products: one is a di-D-fructose-2,6':6,2'-dianhydride (DFAIV) and the other is a levanbiose. The detected levanbiose corresponds to the reaction product from LFTase lacking transferring activity N85S substitution is responsible for the strong enhancement of the hydrolytic activity 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16N238K relative hydrolytic activity: 23.6% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16N85S relative hydrolytic activity: 34.8% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16N85S/A264G relative hydrolytic activity: 49.9% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16N85S/D268E relative hydrolytic activity: 30% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16N85S/N224K/Q229E/T253N relative hydrolytic activity: 37% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.16P313R/F518L relative hydrolytic activity: 15.8% compared to levanbiohydrolase from Microbacterium laevaniformans. Relative hydrolytic activity of wild-type: 10.7% 693593
Results 1 - 9 of 9