EC Number |
Protein Variants |
Reference |
---|
4.1.2.47 | C81A |
about 20% of wild-type activity |
662210 |
4.1.2.47 | C81A |
specific activity is 93% of the wild-type value |
653781 |
4.1.2.47 | C81A |
the mutant is also inhibited by acetate |
704762 |
4.1.2.47 | C81L |
1.3fold increase in kcat for racemic mandelonitrile |
747531 |
4.1.2.47 | C81L |
the mutant has high enantioselectivity towards (R)-mandelonitrile with a kcat value 1.3times higher compared to the wild type enzyme |
747531 |
4.1.2.47 | C81S |
3% of wild-type activity |
662210 |
4.1.2.47 | C81S |
no difference can be observed in the electrophoretic mobilities between the wild-type and mutant protein on native polyacrylamide gels and by isoelectric focusing. Mutation does not change the charge or size of the amino acid side chain, but nevertheless greatly reduces activity |
706644 |
4.1.2.47 | D207A |
mutant protein is insoluble |
728015 |
4.1.2.47 | D208A |
Km-value for 2-hydroxy-2-methylpropanenitrile increases from 101 mM for wild-type enzyme to over 200 mM for the mutant enzyme |
704383 |
4.1.2.47 | E79A |
1% of wild-type activity |
662210 |