EC Number   |
Protein Variants |
Reference |
|---|
    4.1.2.17 | D120N |
mutant with reduced specific activity |
650038 |
| 4.1.2.17 | D25L |
negative effect on catalytic activity and substrate recognition |
652766 |
| 4.1.2.17 | D25T |
negative effect on catalytic activity and substrate recognition |
652766 |
| 4.1.2.17 | D76E |
mutant with reduced specific activity |
650038 |
| 4.1.2.17 | E142Q |
mutant with increased specific activity |
650038 |
| 4.1.2.17 | E73Q |
no enzyme activity |
652834 |
| 4.1.2.17 | E73Q/Y113F/Y209F |
no enzyme activity |
652834 |
| 4.1.2.17 | E73S |
no enzyme activity |
652834 |
| 4.1.2.17 | F131A |
mutant with improved catalyti activity |
728423 |
| 4.1.2.17 | F131A |
the mutant has 1% retroaldol activity with L-fuculose-1-phosphate as compared to the wild type enzyme. The mutant uses (R)-N-benzyloxycarbonyl-prolinal as substrate and is highly stereoselective both with (R)-and with (S)-N-benzyloxycarbonyl-prolinal, exclusively producing the anti- and syn-aldol adducts, respectively |
714742 |
