EC Number |
Protein Variants |
Reference |
---|
4.1.1.43 | E545L |
the mutant shows 730fold decrease in kcat (phenylpyruvate) and 38fold decrease in kcat (indole-3-pyruvate) compared to the wild type enzyme |
714975 |
4.1.1.43 | I335Y |
compared to the wild type enzyme, the mutant exhibits 0.6% catalytic efficiency with phenylpyruvate, 33% catalytic efficiency with 2-oxohexanoic acid, 213% catalytic efficiency with 2-oxopentanoic acid, 1.7% catalytic efficiency with 2-oxobutanoic acid, and 7% catalytic efficiency with pyruvic acid |
714975 |
4.1.1.43 | I335Y/M624W |
compared to the wild type enzyme, the mutant exhibits 0.02% catalytic efficiency with phenylpyruvate, 2.3% catalytic efficiency with 2-oxohexanoic acid, 25% catalytic efficiency with 2-oxopentanoic acid, no activity with 2-oxobutanoic acid, and 59% catalytic efficiency with pyruvic acid |
714975 |
4.1.1.43 | M624W |
compared to the wild type enzyme, the mutant exhibits 3.4% catalytic efficiency with phenylpyruvate, 32% catalytic efficiency with 2-oxohexanoic acid, 131% catalytic efficiency with 2-oxopentanoic acid, 1.4% catalytic efficiency with 2-oxobutanoic acid, and 152% catalytic efficiency with pyruvic acid |
714975 |
4.1.1.43 | more |
an ipdC-knockout mutant produces only 10% (w/v) of the wild type indole-3-acetic acid production level |
680508 |
4.1.1.43 | Q448W |
compared to the wild type enzyme, the mutant exhibits 45% catalytic efficiency with phenylpyruvate, 16% catalytic efficiency with 2-oxohexanoic acid, 231% catalytic efficiency with 2-oxopentanoic acid, 62% catalytic efficiency with 2-oxobutanoic acid, and 130% catalytic efficiency with pyruvic acid |
714975 |