EC Number   |
Protein Variants |
Reference |
|---|
   3.8.1.10 | D10A |
site-directed mutagenesis, the D10A mutant shows over 50% reduced enzyme activity compared to the wild-type enzyme |
732999 |
| 3.8.1.10 | D11A |
site-directed mutagenesis, the D11A mutant shows highly reduced enzyme activity compared to the wild-type enzyme |
732999 |
| 3.8.1.10 | D180A |
site-directed mutagenesis, the D180A mutant shows over 50% reduced enzyme activity compared to the wild-type enzyme |
732999 |
| 3.8.1.10 | D180A |
the mutation destabilizes the rotation of the nucleophile D10, fixes catalytic water around D10, and prevents K151 from approaching D10 |
699200 |
| 3.8.1.10 | D181A |
the activity of mutant is similar to wild-type enzyme |
-, 648284 |
| 3.8.1.10 | D181E |
the activity of mutant is similar to wild-type enzyme |
-, 648284 |
| 3.8.1.10 | D181R |
the activity of mutant is similar to wild-type enzyme |
-, 648284 |
| 3.8.1.10 | D184A |
site-directed mutagenesis, the mutant cannot be expressed recombinantly |
732999 |
| 3.8.1.10 | D185A |
site-directed mutagenesis, the mutant cannot be expressed recombinantly |
732999 |
| 3.8.1.10 | D194N |
site-directed mutagenesis |
733212 |
