EC Number   |
Protein Variants |
Reference |
|---|
   3.5.99.7 | E295D |
site-directed mutagesis, mutant kinetics compared to the wild-type, overview |
-, 718894 |
| 3.5.99.7 | E295S |
by site-directed mutagenesis, the Pseudomonas putida UW4 single mutant is constructed using pET30a (+) with the full-length ACC deaminase as the template |
-, 694780 |
| 3.5.99.7 | E295S/L322T |
the double mutant is constructed using the E295S mutant as the template |
-, 694780 |
| 3.5.99.7 | E295S/L322T |
the mutations lead to a loss of enzyme activity against 1-aminocyclopropane-1-carboxylate |
-, 735145 |
| 3.5.99.7 | G44D |
completely inactive, CD spectrum is identical to wild-type |
-, 667784 |
| 3.5.99.7 | K51T |
crystal structure in complex with substrate |
648271 |
| 3.5.99.7 | more |
ACC deaminase enhances Agrobacterium tumefaciens-mediated gene transfer into plant cells, e.g. of Cucumis melo var. cantaloupensis or Arabidopsis thaliana, by converting the direct precursor for ethylene, which inhibits gene transfer, into 2-oxobutanoate, overview |
-, 684575 |
| 3.5.99.7 | more |
acdS gene deletion mutagenesis, Sinorhizobium sp. BL3-enhancing ACC deaminase activity (BL3+) and defective mutant (BL3-) strains are constructed in order to investigate the effects of this enzyme on symbiosis and nodule senescence. Modulation competitiveness is weaker in BL3- than in the wild-type, but is stronger in BL3+. The inoculation of BL3- into mung bean results in less plant growth, a lower nodule dry weight, and smaller nodule number than those in the wild-type, whereas the inoculation of BL3+ has no marked effects |
757612 |
| 3.5.99.7 | more |
compared with the wild-type A1501, the acdS mutant A1815 is more sensitive to the environmental stresses of salt and heavy metal shock. The survival rate of A1815 is lowered by 1-2 orders of magnitude compared with the wild-type when exposed to 1.0 M NaCl treatment for 3 h and by 2-3 orders of magnitude when exposed to 13.2 mM NiCl2 for 3 h |
-, 757414 |
| 3.5.99.7 | more |
construction and complementation of acdS and lrpL mutant strains with wild-type strain D3 ACC deaminase, phenotypes, overview |
-, 719206 |
