EC Number   |
Protein Variants |
Reference |
|---|
   3.5.4.42 | D188A |
about 6fold increase in kcat/Km value |
739428 |
| 3.5.4.42 | D188N |
activity similar to wild-type |
739428 |
| 3.5.4.42 | D303A |
activity similar to wild-type |
739428 |
| 3.5.4.42 | D303N |
activity similar to wild-type |
739428 |
| 3.5.4.42 | degradation |
microorganisms EAA-3 and EAA-4 degrade atrazine substrate at 0.42 mg per l and h in 60 h at 99% efficiency.With glucose supplementation, the overall degradation rates determined for EAA-3 and EAA-4 are 0.42 and 0.40 mg per h and l, respectively. The strains metabolize 2-hydroxyatrazine, desethylatrazine and desisopropylatrazine, but degradation of desisopropyl-2-hydroxyatrazine and desisopropyl desethyl-2-hydroxyatrazine is only observed in presence of glucose |
-, 739711 |
| 3.5.4.42 | H219A |
4fold increase in kcat value |
739428 |
| 3.5.4.42 | H249A |
substitution of an active site histidine residue, complete loss of activity |
739428 |
| 3.5.4.42 | K65A |
more than 15fold increase in kcat/Km value, 30fold increase in kcat for ammelide |
739428 |
| 3.5.4.42 | K65R |
more than 15fold increase in kcat/Km value, 12fold increase in kcat for ammelide |
739428 |
| 3.5.4.42 | N304A |
slight increase in kcat for ammelide |
739428 |
