EC Number   |
Protein Variants |
Reference |
|---|
    3.5.4.13 | E138A |
no enzymic activity, no change in overall structure compared to wild-type |
669375 |
| 3.5.4.13 | E138A |
site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered |
686667 |
| 3.5.4.13 | E138D |
site-directed mutagenesis, the mutant enzyme shows a 140fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme |
684700 |
| 3.5.4.13 | E138Q |
no enzymic activity, no change in overall structure compared to wild-type |
669375 |
| 3.5.4.13 | H121A |
site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered |
686667 |
| 3.5.4.13 | R115A |
no enzymic activity, no change in overall structure compared to wild-type |
669375 |
| 3.5.4.13 | R115Q |
site-directed mutagenesis, inactive mutant |
684700 |
| 3.5.4.13 | S111C |
site-directed mutagenesis, inactive mutant |
684700 |
| 3.5.4.13 | S111T |
site-directed mutagenesis, the mutant enzyme shows a 30fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme, modeling of the active site of the S111T enzyme, overview |
684700 |
| 3.5.4.13 | V122G |
site-directed mutagenesis, inactive mutant |
686667 |
