EC Number   |
Protein Variants |
Reference |
|---|
   3.5.1.77 | A164T |
mutant, screening for thermostable enzyme |
695822 |
| 3.5.1.77 | A18T/Y30N/K34E |
kinetic properties and thermodynamic parameters of the mutant enzyme are identical with those of the wild-type enzyme. More than 80% improve in solubility compared to wild-type enzyme |
684966 |
| 3.5.1.77 | A222C |
crystal structure is nearly identical to wild-type enzyme, half-life at 50°C is 1.1fold higher than that of wild-type enzyme |
669884 |
| 3.5.1.77 | A302C |
crystal structure is nearly identical to wild-type enzyme, 123.9% of wild-type activity, temperature-optimum is 10°C higher than that of wild-type enzyme, half-life at 50°C is 2.5fold higher than that of wild-type enzyme, 4.2fold increase in kcat/Km at 65°C, 1.5fold increase at 55°C and 1.15fold increase at 37°C |
669884 |
| 3.5.1.77 | A36E |
mutant, screening for thermostable enzyme |
695822 |
| 3.5.1.77 | A36V |
mutant, screening for thermostable enzyme |
695822 |
| 3.5.1.77 | C172A |
inactive mutant enzyme |
656143 |
| 3.5.1.77 | C172A |
mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity |
-, 31892 |
| 3.5.1.77 | C172S |
less than 0.1% of the wild-type enzyme |
656143 |
| 3.5.1.77 | C172S |
mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity |
-, 31892 |
