EC Number   |
Protein Variants |
Reference |
|---|
    3.5.1.54 | G559E/G572E |
site-directed mutagenesis, crystal structure analysis, the mutant shows 14fold increaed Km for allophanate, and reduced substrate binding at the N-domain active site, but is catalytically active |
734220 |
| 3.5.1.54 | G559E/G572E |
site-directed mutagenesis, the mutant shows abolished activity |
-, 753037 |
| 3.5.1.54 | H488A |
site-directed mutagenesis |
752542 |
| 3.5.1.54 | H488A |
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme below pH 8.0, but slightly reduced activity above pH 8.0 |
733179 |
| 3.5.1.54 | K91A |
site-directed mutagenesis, inactive mutant |
669098 |
| 3.5.1.54 | more |
construction of the gene encoding C-terminally truncated AtzF mutant, AtzF467 |
733034 |
| 3.5.1.54 | more |
construction of the gene encoding C-terminally truncated AtzF mutant, AtzF467, the mutant shows similar activity as the wild-type enzyme below pH 8.0, but slightly reduced activity above pH 8.0 |
733179 |
| 3.5.1.54 | more |
generation of isolated domains |
734220 |
| 3.5.1.54 | more |
generation of the KlUADELTABCCP construct via insertion a stop codon after base pair 5223 |
-, 753037 |
| 3.5.1.54 | R307A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
-, 733342 |
