EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.68 | C461S |
site-directed mutagenesis, ELS1C461S is not capable of cleaving the extension oV the carboxyl terminus of SUMO1 |
718246 |
| 3.4.22.68 | C517S |
catalytically inactive |
-, 755162 |
| 3.4.22.68 | C580S |
site-directed mutagenesis, generation of a catalytically inactive mutant of Ulp1, the mutant is greatly enriched at the septin ring of dividing yeast cells. The 218-amino acid, substrate-trapping mutant of the catalytic domain of Ulp1 is necessary and sufficient for septin localization |
717436 |
| 3.4.22.68 | D451N |
the mutation destroys an essential salt bridge formed between Smt3 and Ulp1 |
717436 |
| 3.4.22.68 | D451N/C580S |
site-directed mutagenesis, abolished accumulation of the full-length Ulp1 double-mutant at the septin ring |
717436 |
| 3.4.22.68 | I435V/N450S/I504T/C580S |
site-directed mutagenesis, the mutant shows a reduced ability to enrich at the septin ring |
717436 |
| 3.4.22.68 | K691A |
site-directed mutagenesis of SENP7 |
717852 |
| 3.4.22.68 | K691E |
site-directed mutagenesis of SENP7 |
717852 |
| 3.4.22.68 | more |
a genetic mutation inactivating all three gene products of SENP2 is generated by alternative splicing |
714673 |
| 3.4.22.68 | more |
generation of a collection of GFP-tagged Ulp1 truncations and domains that were expressed under control of the Ulp1 promoter. truncations and domains of Ulp1, that retain substrate targeting information, also localize to the septin ring in G2/M-arrested cells. Usage of the targeting and SUMO-binding properties of Ulp1(3)(C580S) to purify Smt3-modified proteins from cell extracts. Deletion of the entire SBS domain on the localization of Ulp1(3)(C580S). The Ulp1(3)(C580S)SBSDELTA construct does not localize to the septin ring in the majority of cells |
717436 |
