EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.49 | C1129S |
inactive |
709220 |
| 3.4.22.49 | C2029A |
- |
638869 |
| 3.4.22.49 | C2029A |
no autoproteolytic cleavage, unable to cleave SCC1 |
638865 |
| 3.4.22.49 | E1483R/R1486E |
autoproteolytically cleaved like wild-type |
638869 |
| 3.4.22.49 | E1503R/R1506E |
autoproteolytically cleaved like wild-type |
638869 |
| 3.4.22.49 | E1532R/R1535E |
autoproteolytically cleaved like wild-type |
638869 |
| 3.4.22.49 | H1531A |
active site point mutation prevents Scc1 from being cleaved after binding |
638864 |
| 3.4.22.49 | more |
analysis of the homozygous zebrafish mutant cds (cease&desist), bearing a mutation in the separase gene, reveals high levels of polyploidy and aneuploidy, spindle defects, and a mitotic exit delay in mutant embryos. Carcinogenesis studies demonstrate that cds heterozygous adults have an eightfold increase in the percentage of fish bearing epithelial tumors |
680013 |
| 3.4.22.49 | more |
clear evidence for a non-proteolytic function of separase is provided: by separase gene deletion in mouse oocytes it is shown that separase null-oocytes are unable to either destroy chesin along chromosome arms or segregate homologous chromosomes and these oocytes are unable to complete cell division. Microinjection of wild-type separase mRNA in separase null-oocytes restores normal homologue disjunction and polar body extrusion |
682065 |
| 3.4.22.49 | more |
generation of human cells with one hESP allele-encoding uncleavable protein and another allele harboring a single cleavage site, the cells grow slowly owing to cell cycle delay, in particular during G2/M transition, but not when it was expected, i.e. during anaphase |
718194 |
