EC Number |
Protein Variants |
Reference |
---|
3.4.21.50 | H210/W169F |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 8.6% of the wild-type ratio |
651129 |
3.4.21.50 | H210A |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 7.1% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is identical to the wild-type value |
651790 |
3.4.21.50 | H210A |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 80% of the wild-type ratio |
651129 |
3.4.21.50 | H210A/W169A |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 0.25% of the wild-type ratio |
651129 |
3.4.21.50 | H210F |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 14% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 29.5% of the wild-type value |
651790 |
3.4.21.50 | H210K |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 0.02% of the wild-type ratio |
651129 |
3.4.21.50 | H210S |
crystallization data. Optimum pH value shifts from around pH 9 of wild-type to approximately pH 7, while retaining high activity |
717034 |
3.4.21.50 | H210S |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin is 8.6% of the wild-type value, the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin is 1.7fold higher than the wild-type value |
651790 |
3.4.21.50 | H210S |
the ratio of turnover number to Km-value for tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin as substrate is 1.7fold higher than the wild-type ratio |
651129 |
3.4.21.50 | more |
generation of a Gly681-Ala710 deletion mutant |
-, 732885 |