EC Number   |
Protein Variants |
Reference |
|---|
   3.4.17.8 | C115S |
site-directed mutagenesis, truncated mutant of PBP5, pH-dependency, kinetics, and activity compared to the wild-type enzyme |
683158 |
| 3.4.17.8 | D344S |
the mutant shows highly reduced activity compared to the wild-type |
683630 |
| 3.4.17.8 | D59A |
73% decrease in ratio kcat/KM for D-Ala-D-Ala substrate, EC3.4.13.B1 activity |
-, 668456 |
| 3.4.17.8 | D59S |
strong increase in ratio kcat/KM for EC 3.4.17.8 activity, 50% increase in ratio kcat/KM for D-Ala-D-Ala substrate, EC3.4.13.B1 activity |
-, 668456 |
| 3.4.17.8 | K172N/R173N |
site-directed mutagenesis, M1 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview |
683602 |
| 3.4.17.8 | K199Q |
site-directed mutagenesis, M5 mutant, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1 |
683602 |
| 3.4.17.8 | K199Q/K203Q |
site-directed mutagenesis, M2 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview |
683602 |
| 3.4.17.8 | K203Q |
site-directed mutagenesis, M3 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview |
683602 |
| 3.4.17.8 | K213C/C115S |
site-directed mutagenesis, truncated double mutant of PBP5, pH-dependency, kinetics, and activity compared to the wild-type enzyme |
683158 |
| 3.4.17.8 | K47C/C115S |
site-directed mutagenesis, truncated double mutant of PBP5, pH-dependency, kinetics, and activity compared to the wild-type enzyme |
683158 |
