EC Number   |
Protein Variants |
Reference |
|---|
    3.4.13.22 | C78S/C157S |
site-directed mutagenesis, reduced activity |
649823 |
| 3.4.13.22 | D123A |
site-directed mutagenesis, inactive, nearly no remaining Zn2+ |
649823 |
| 3.4.13.22 | D145A/E146A |
site-directed mutagenesis, 6fold lowered kcat compared to wild-type, 11% reduced zinc content compared to wild-type |
649823 |
| 3.4.13.22 | D59A |
73% decrease in ratio kcat/KM for D-Ala-D-Ala substrate |
-, 668456 |
| 3.4.13.22 | D59S |
50% increase in ratio kcat/KM for D-Ala-D-Ala substrate |
-, 668456 |
| 3.4.13.22 | E181A |
site-directed mutagenesis, inactive, 21.8% reduced zinc content compared to wild-type |
649823 |
| 3.4.13.22 | H116A |
site-directed mutagenesis, inactive, 92.1% reduced zinc content compared to wild-type |
649823 |
| 3.4.13.22 | H116A/H184A |
site-directed mutagenesis, inactive, 97.5% reduced zinc content compared to wild-type |
649823 |
| 3.4.13.22 | H13A |
site-directed mutagenesis, activity equivalent to wild-type, 8.3% reduced zinc content compared to wild-type |
649823 |
| 3.4.13.22 | H149A |
site-directed mutagenesis, equivalent to wild-type |
649823 |
