EC Number   |
Protein Variants |
Reference |
|---|
   3.2.2.29 | D133A |
the mutation significantly reduces the enzymes ability to enhance Wnt signaling |
732173 |
| 3.2.2.29 | H151A |
the mutant shows increased transition rates and activity compared to the wild type enzyme |
751387 |
| 3.2.2.29 | H151A |
the mutation results in a 2fold decrease in enzymatic activity |
751393 |
| 3.2.2.29 | more |
construction of a His-tagged truncated Thd1p variant comprising residues 650M-1063N from Escherichia coli strain BL21 (DE3) |
694421 |
| 3.2.2.29 | N140A |
inactive |
732173, 732556 |
| 3.2.2.29 | N140A |
site-directed mutagenesis, the mutant variant binds substrate DNA with the same tight affinity as wild-type TDG, but it has no detectable base excision activity for a G:T substrate, and its excision rate is vastly diminished for G:U, G:FU, i.e. fluorouridine, and G:BrU, i.e. bromodeoxyuridine, substrates. Altered kinetics compared to the wild-type enzyme, overview |
709075 |
| 3.2.2.29 | N140A |
the mutant exhibits 1800fold and more than 20000fold reduced activity at pH 6.0 after 24 h for guanine:uracil and after 72 h for guanine:5-carboxylcytosine, respectively |
731706 |
| 3.2.2.29 | N140A |
the mutation completely depletes guanine-thymine glycosylase activity and causes a huge (27000fold) loss in guanine-uracil activity |
751726 |
| 3.2.2.29 | N140A |
the mutation results in a decrease of enzymatic activity |
751393 |
| 3.2.2.29 | N140A/R275L |
site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, overview |
709075 |
