EC Number |
Protein Variants |
Reference |
---|
3.2.1.57 | D353N |
enzyme shows no activity with pullulan and panose as substrate |
664832 |
3.2.1.57 | D372N |
enzyme shows no activity with pullulan and panose as substrate |
664832 |
3.2.1.57 | D373N |
enzyme shows no activity with pullulan and panose as substrate |
664832 |
3.2.1.57 | E273N |
45% of wild-type activity with pullulan as substrate, 74% of wild-type activity with panose as substrate |
664832 |
3.2.1.57 | E356Q |
38% of wild-type activity with pullulan as substrate, 50% of wild-type activity with panose as substrate |
664832 |
3.2.1.57 | N448A |
site-directed mutagenesis, glycan-deficient variant, Asn448 is not N-glycosylated, the mutant shows reduced thermal stability compared to the wild-type enzyme |
-, 732013 |
3.2.1.57 | S450A |
site-directed mutagenesis, glycan-deficient variant, by replacing Ser450 with Ala, Asn448 is not N-glycosylated, but the Asn448 side chain remains intact, unlike the Asn448Ala mutation, the mutant shows reduced thermal stability compared to the wild-type enzyme |
-, 732013 |
3.2.1.57 | W240F |
130% of wild-type activity with pullulan as substrate, 160% of wild-type activity with panose as substrate |
664832 |
3.2.1.57 | W31F |
38% of wild-type activity with pullulan as substrate, 140% of wild-type activity with panose as substrate |
664832 |
3.2.1.57 | W32F |
90% of wild-type activity with pullulan as substrate, 120% of wild-type activity with panose as substrate |
664832 |