EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.38 | D206N |
catalytically active mutant enzyme, similar temperature optimum like wild-type enzyme. The high-catalytic turn-over rate by D206N for beta-glucosidase activity makes it a useful enzyme in cellulose degradation at high temperatures |
750443 |
| 3.2.1.38 | D206Q |
mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides |
750443 |
| 3.2.1.38 | E173N |
mutant enzyme shows no activity |
751822 |
| 3.2.1.38 | E205A |
complete loss of activity |
-, 714243 |
| 3.2.1.38 | E207S |
no hydrolytic activity |
750443 |
| 3.2.1.38 | E239A |
complete loss of activity |
-, 714243 |
| 3.2.1.38 | E389N |
mutant enzyme shows no activity |
751822 |
| 3.2.1.38 | E399S |
no hydrolytic activity |
750443 |
| 3.2.1.38 | E446N |
beta-fucosidase activity is 26.49% compared to wild-type activity, beta-glucosidase activity is 21.37% compared to wild-type activity, beta-galactosidase activity is 15.7% compared to wild-type activity |
751822 |
| 3.2.1.38 | more |
an overproducing mutant is isolated by UV mutagenesis and screening on agar plates containing X-Gal substrate. Cytoplasmic extracts of the mutant contain 25fold higher enzyme levels than the parent |
-, 727060 |
