EC Number |
Protein Variants |
Reference |
---|
3.2.1.156 | D128A |
specific activity is 290fold lower than that of the wild-type enzyme |
-, 659431 |
3.2.1.156 | D148A |
site-directed mutagenesis, inactive mutant |
-, 731182 |
3.2.1.156 | D263A |
conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose |
665712 |
3.2.1.156 | D263A |
specific activity is 4421fold lower than that of the wild-type enzyme |
-, 659431 |
3.2.1.156 | D263C |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
708955 |
3.2.1.156 | D263C |
conversion of glycosynthase from converting enzyme, synthesis of xylotriose from xylobiose + HF + xylose, highest glycosynthase activity of mutants tested |
665712 |
3.2.1.156 | D263C |
saturation mutagenesis, the mutant accumulates high amounts of xylotriose, but reduced F- releasing activity compared to the wild-type enzyme |
710649 |
3.2.1.156 | D263G |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
708955 |
3.2.1.156 | D263G |
conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose |
665712 |
3.2.1.156 | D263L |
conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose |
665712 |