EC Number |
Protein Variants |
Reference |
---|
3.2.1.130 | C188A |
enzymatic activity can not be fully rescued with a back-engineered Cys188Ala CHO cell endomannosidase |
679134 |
3.2.1.130 | C188S |
enzymatic activity can not be fully rescued with a back-engineered Cys188Ser CHO cell endomannosidase |
679134 |
3.2.1.130 | C188W |
enzyme activity can be reversed in cells with a back-engineered Cys188Trp CHO cell endomannosidase, in particular N-glycans of alpha1-antitrypsin become fully processed |
679134 |
3.2.1.130 | E154A |
the mutant shows near zero activity |
-, 732816 |
3.2.1.130 | E329A |
the mutant shows near zero activity |
-, 732816 |
3.2.1.130 | E332A |
the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme |
-, 732816 |
3.2.1.130 | E333Q |
inactive |
731659 |
3.2.1.130 | E407D |
the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)-Man |
750314 |
3.2.1.130 | more |
in CHO-K1 cells expressing DELTAsig-rEndo, lacking the whole signal sequence, or DELTATMD-rEndo, lacking the transmembrane domain, endomannosidase is undetectable. DELTACT-rEndo, lacking the cytoplasmic tail, exhibits an ER localization and fails to maintain a type II membrane topology |
697210 |
3.2.1.130 | W188C |
molecular defect in CHO cells, is functionally important since it alone results in endoplasmic reticulum mislocalization of endomannosidase and causes greatly reduced in vivo activity |
679134 |