EC Number   |
Protein Variants |
Reference |
|---|
   3.2.1.129 | D138A |
lower Km than wild-type enzyme |
646722 |
| 3.2.1.129 | D154A |
lower Km than wild-type enzyme |
646722 |
| 3.2.1.129 | D489N |
back-mutations, partial reduction of enzymic activity. Single amino acid substitution do not inactivate the catalytic activity totally |
678066 |
| 3.2.1.129 | DELTA DI |
insoluble enzyme |
646722 |
| 3.2.1.129 | DELTA DII |
insoluble enzyme |
646722 |
| 3.2.1.129 | DELTA P-loop |
higher Km than wild-type enzyme |
646722 |
| 3.2.1.129 | E581A |
1.8% relative activity compared to the wild type enzyme |
705185 |
| 3.2.1.129 | E581A |
behaves like wild-type in terms of expression level, maturation by proteolytic cleavage and trimer formation |
666430 |
| 3.2.1.129 | E581A |
the mutation results in complete loss of sialidase activity |
704497 |
| 3.2.1.129 | G153A |
less turnover than wild-type enzyme |
646722 |
