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EC Number
Protein Variants
Commentary
Reference
3.2.1.125
E186D
inactive
713606
3.2.1.125
E186Q
inactive
713606
3.2.1.125
E186Q/E420Q
inactive
713606
3.2.1.125
E420Q
the mutant shows 0.5% activity with raucaffricine compared to the wild type enzyme
713606
3.2.1.125
E476A
the mutant shows 0.57% activity with raucaffricine compared to the wild type enzyme
713606
3.2.1.125
E476L
the mutant shows 0.67% activity with raucaffricine compared to the wild type enzyme
713606
3.2.1.125
F485Y
the mutant shows 24.24% activity with raucaffricine compared to the wild type enzyme
713606
3.2.1.125
H193A
the mutant shows 43.66% activity with raucaffricine compared to the wild type enzyme
713606
3.2.1.125
S390G
the mutant shows 30.91% activity with raucaffricine compared to the wild type enzyme
713606
3.2.1.125
T189A
the mutant shows 63.94% activity with raucaffricine compared to the wild type enzyme
713606
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