EC Number |
Protein Variants |
Reference |
---|
3.1.6.19 | F250G |
190fold reduction in catalytic activity |
761062 |
3.1.6.19 | H317A |
1300fold decrease in kcat, whereas Km is only slightly increased |
761062 |
3.1.6.19 | S233F/F250G |
three-fold to four-fold times lower kcat value and a 10fold higer Km value, variant displays activity towardstowards (S)-2-octyl sulfate |
761062 |
3.1.6.19 | S233F/F250S |
about 30% of wild-type activity |
761062 |
3.1.6.19 | S233Y |
2.5fold reduction in catalytic activity |
761062 |
3.1.6.19 | S233Y/F250G |
three-fold to four-fold times lower kcat value |
761062 |
3.1.6.19 | Y417D |
inactive |
761062 |
3.1.6.19 | Y417F |
114fold decrease in kcat value |
761062 |
3.1.6.19 | Y417H |
238 fold decrease in kcat value |
761062 |