EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.36 | C383S |
mutant with an almost entirely eliminated 3-phosphatase and 4-phosphatase activity, but a maintained 5-phosphatase activity also fails to rescue the endocytic defects in synj1-/- neurons |
682125 |
| 3.1.3.36 | C392S |
site-directed mutagenesis, inactivating mutation in the conserved CX5R(T/S) motif of the Sac domain |
716022 |
| 3.1.3.36 | C641A |
site-directed mutagenesis, the mutation removes the prenylation site of the enzyme |
716022 |
| 3.1.3.36 | C910A |
mutation of the C-terminal cysteine (C910A), which abolishes prenylation of INPP5B does not affect cellular targeting of INPP5B |
681014 |
| 3.1.3.36 | D192A |
to assess the role of catalytic activity of SKIP on its suppressive effect, a phosphatase-negative mutant D192A is generated: 5-phosphatase activity is not required for the suppressive effect |
691698 |
| 3.1.3.36 | D193/E195A |
binding activity is much lower compared to wild-type fragment consisting of amino acids 124-314. Binding activity for active RhoA is dramatically reduced compared to wild-type |
732478 |
| 3.1.3.36 | D223/K224A |
binding activity is much lower compared to wild-type fragment consisting of amino acids 124-314 |
732478 |
| 3.1.3.36 | D319A |
catalytically inactive mutant |
691699 |
| 3.1.3.36 | D319A |
inactive mutant bearing a mutation in 5-phosphatase catalytic domain. This mutant is not able to restore dd5p4-null cells |
682938 |
| 3.1.3.36 | D360A/N362A |
significant decrease in catalytic activity |
751562 |
