EC Number |
Protein Variants |
Reference |
---|
3.1.21.8 | A83L |
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity |
727747 |
3.1.21.8 | E118A |
complete loss of cleavage activity |
727747 |
3.1.21.8 | E118A |
crystallization data |
715906 |
3.1.21.8 | E118A |
mutation in a putatively catalytic residue. Mutant E118A is able to bind stably as monomer or dimer to a single substrate. In low-salt conditions, mutant is present as dimer in solution |
728389 |
3.1.21.8 | G49A |
complete loss of cleavage activity |
727747 |
3.1.21.8 | G49A |
in low-salt conditions, mutant is present as dimer in solution |
728389 |
3.1.21.8 | I24A |
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity |
727747 |
3.1.21.8 | K12A |
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity |
727747 |
3.1.21.8 | K76A |
much reduced catalytic activity |
727747 |
3.1.21.8 | L16A |
similar to wild-type, cleavage activity is 20 to 70% of the nicking activity |
727747 |