EC Number   |
Protein Variants |
Reference |
|---|
   3.1.12.1 | C188A |
the mutant protein is inactive in both binding and cleavage of ssDNA. It forms various protein aggregates |
-, 725211 |
| 3.1.12.1 | C191A |
the mutant protein is inactive in both binding and cleavage of ssDNA. It forms various protein aggregates |
-, 725211 |
| 3.1.12.1 | C32A |
the mutant protein is inactive in both binding and cleavage of ssDNA. It forms various protein aggregates |
-, 725211 |
| 3.1.12.1 | D99A |
inactive mutant enzyme |
-, 725211 |
| 3.1.12.1 | D99A |
the mutant enzyme fails to cut DNA |
-, 726307 |
| 3.1.12.1 | more |
cas4-2 dene deletion |
751558 |
| 3.1.12.1 | more |
determining genetic requirement for in vivo adaptation by analyzing mutant strains lacking cas4-1 and cas4-2 individually or simultaneously, phenotypes. Adaptation is reduced, but not completely prevented, by deletion of either or both of the cas4 genes. Strikingly, longer spacers around 40-70 base pairs (bp) in length are acquired in the double deletion strain as opposed to only about 37 bp in wild-type |
751557 |
