EC Number   |
Protein Variants |
Reference |
|---|
   3.1.11.5 | D1067A |
chi is not a hot-spot for recombination in the mutant, the mutant is sligthtly defective for recombinational repair |
653269 |
| 3.1.11.5 | D1067A |
The mutation in the carboxyl-terminal nuclease domain of the RecB subunit abolishes nuclease activity on both single- and double-stranded DNA but the mutant enzyme is active as helicase. The mutant is unable to produce chi-specific fragments from either strand of a chi-containing double-stranded DNA substrate. |
652076 |
| 3.1.11.5 | D1080A |
chi is not a hot-spot for recombination in the mutant, the mutant is sligthtly defective for recombinational repair |
653269 |
| 3.1.11.5 | D1080A |
Comparison: recB(D1080A)CD is recombinant-deficient, and sensitive to DNA damaging agents, and the purified enzyme failed to load RecA during DNA winding. recB(D1080A)C is recombinant-proficient and resistant to DNA damaging agents, and the mutant is active RecA loading assay. |
653653 |
| 3.1.11.5 | D1118A |
site-directed mutagenesis, inactivation in the nuclease center of RecB |
-, 716691 |
| 3.1.11.5 | K1082A |
chi is not a hot-spot for recombination in the mutant, the mutant is sligthtly defective for recombinational repair |
653269 |
| 3.1.11.5 | K1082Q |
The mutation in the carboxyl-terminal nuclease domain of the RecB subunit abolishes nuclease activity on both single- and double-stranded DNA but the mutant enzyme is active as helicase. The mutant is unable to produce chi-specific fragments from either strand of a chi-containing double-stranded DNA substrate. |
652076 |
| 3.1.11.5 | K177Q |
involved in ATP binding site of the recD protein |
134029 |
| 3.1.11.5 | K229Q |
site-directed mutagenesis, inactivation of the ATPase active site of RecD |
-, 716691 |
| 3.1.11.5 | K29Q |
site-directed mutagenesis, inactivation of the ATPase active site of RecB |
-, 716691 |
