EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.58 | D115A |
decreased catalytic activity, 6.4% of wild-type activity on N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine-adhesin |
-, 698581 |
| 3.1.1.58 | D48A |
site-directed mutagenesis of the catalytic residue abolishes the esterase activity of the enzyme, the mutant strain is deficient in holdfast anchoring to the cell body, and small holdfasts are shed into the medium. The mutant enzyme has a similar secondary structure compared to the wild-type enzyme based on circular dichroism measurement |
730413 |
| 3.1.1.58 | H184A |
mutant, deficient in biofilm formation |
-, 698581 |
| 3.1.1.58 | more |
deletion of gene hfsH abolishing surface adhesion of the cell. The gene hfsH deletion mutant synthesizes defective holdfasts with low adhesiveness and cohesiveness that cannot be anchored to the cell body. In contrast, overexpression of HfsH increases cell adherence |
730413 |
| 3.1.1.58 | more |
deletion of gene hfsH abolishing surface adhesion of the cell. The gene hfsH deletion mutant synthesizes defective holdfasts with low adhesiveness and cohesiveness that cannot be anchored to the cell body. In contrast, overexpression of HfsH increases cell adherence. Phenotypes of Asticcacaulis biprosthecum transposon mutants with insertions in a number of hfs genes, overview |
730413 |
| 3.1.1.58 | more |
enzyme deletion by transposon mutagenesis of gene icaB |
729238 |
| 3.1.1.58 | PgaB271 |
DUF187 domain truncation mutant, amino acids 1-271, deficient in biofilm formation and poly-beta-1,6-N-acetyl-D-glucosamine secretion, 4.1% of wild-type activity on N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine-adhesin |
-, 698581 |
| 3.1.1.58 | PgaB410 |
DUF187 domain truncation mutant, amino acids 1-410, proteolytic cleavage during overexpression |
-, 698581 |
| 3.1.1.58 | PgaB516 |
DUF187 domain truncation mutant, amino acids 1-516, deficient in biofilm formation and poly-beta-1,6-N-acetyl-D-glucosamine secretion, 5% of wild-type activity on N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine-adhesin, proteolytic cleavage during overexpression |
-, 698581 |
