EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.43 | D123N |
point mutant |
693224 |
| 3.1.1.43 | D338A |
0.07% of wild-type kcat |
652282 |
| 3.1.1.43 | H370A |
0.07% of wild-type kcat |
652282 |
| 3.1.1.43 | H610A |
more than 69% of wild-type kcat, partially purified |
652282 |
| 3.1.1.43 | more |
based on the crystal structure of the AEH and cefprozil complex, 13 residues not directly involved in substrate recognition are mutated individually, mutant screening for activity using cefprozil as a model product, overview |
729461 |
| 3.1.1.43 | more |
synthesis of amino-betalactam antibiotic cephalexin by the recombinant enzyme produced in Escherichia coli VKPM B-11246 is more efficient in comparison with the wild-type enzyme prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. Addition of ethylene glycol at 33 vol% to the reaction medium for cephalexin synthesis improves the yield from 70% to 95% |
-, 730436 |
| 3.1.1.43 | more |
Xanthomonas citri strain K24, a beta-lactamase deficient IFO 3835 mutant strain is active at pH 6.0-7.0 in cephalexin synthesis in 5% vol 2-butanol |
-, 729021 |
| 3.1.1.43 | R85A |
67% of wild-type kcat |
652282 |
| 3.1.1.43 | R87I |
site-directed saturation mutagenesis |
729461 |
| 3.1.1.43 | S122A |
point mutant |
693224 |
