EC Number |
Protein Variants |
Reference |
---|
3.1.1.17 | E48A |
mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme |
693673 |
3.1.1.17 | more |
SMP30/GNL knockout mice lack the ability to synthesize vitamin C in vivo |
690769 |
3.1.1.17 | more |
the potential gluconolactone production of the gluconolactonase-deficient strain Zymomonas mobilis ZM4 gnlDELTA, which has completely lost its gluconolactonase activity, are investigated via growth inhibitory assay and biotransformation of glucose and fructose into gluconolactone and sorbitol, respectively. The mutant strain shows severe growth inhibitions in the presence of 2 mM hydrogen peroxide, indicating that it is more susceptible to oxidative stress conditions than the wild-type strain |
-, 749689 |
3.1.1.17 | N134A |
mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme |
693673 |
3.1.1.17 | N191 |
mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme |
693673 |
3.1.1.17 | synthesis |
the enzyme is useful in the enzymatic bioconversion of fructose and lactose to gluconic acid and sorbitol, respectively, by the enzyme's glucose-fructose oxidoreductase and gluconolactonase activities, method development and evaluation for separation of reaction product mixtures containing lactobionic acid, sorbitol, lactose and fructose by HPLC, overview |
681053 |