EC Number   |
Protein Variants |
Reference |
|---|
    2.9.1.2 | A239T |
naturally occurring mutation and site-directed mutagenesis. The mutant forms a stable complex with GroEL. Residue Ala239 is located in helix alpha8 near the site that interacts with the variable arm of tRNASec, and is distant from the active site. The A239T variant binds tRNASec with less affinity compared to wild-type SepSecS, but its catalytic function is unaffected |
762417 |
| 2.9.1.2 | H166A |
the mutant is partially active in forming Sec-tRNASec in vivo. In vitro, the mutant is partially active in forming Cys-tRNASec |
694428 |
| 2.9.1.2 | H166F |
mutant is inactive in vivo |
694428 |
| 2.9.1.2 | H166Q |
mutant is inactive in vivo |
694428 |
| 2.9.1.2 | K173A |
in vivo activity of the mutant is indistinguishable from that of the wild-type enzyme |
695136 |
| 2.9.1.2 | K173M |
in vivo activity of the mutant is indistinguishable from that of the wild-type enzyme |
695136 |
| 2.9.1.2 | more |
mapping pathogenic mutations onto the tetrameric human SepSecS-tRNASec complex, overview |
762417 |
| 2.9.1.2 | Q105A |
mutant is inactive in vivo |
695136 |
| 2.9.1.2 | R307A |
the mutant is significantly less active in L-selenocysteinyl-tRNASec formation in vivo and Cys-tRNASec formation in vitro |
694428 |
| 2.9.1.2 | R313A |
mutant is inactive in vivo |
695136 |
