EC Number |
Protein Variants |
Reference |
---|
2.8.1.7 | C314S |
loss of actalytic activity |
761791 |
2.8.1.7 | C325A |
no cysteine desulfurase activity |
645636 |
2.8.1.7 | C326A |
inactive,the mutant enzyme is incapable of nucleophilic attack on the sulfur of the substrate L-cysteine |
702269 |
2.8.1.7 | C327S |
inactive |
726296 |
2.8.1.7 | C327S |
no residual cysteine desulfurase activity, mutant is not able to reactivate apo-Fe DndC protein |
672260 |
2.8.1.7 | C327S |
sited-directed mutagenesis, no cysteine desulfurase activity, no ability to reactivate the apo-Fe DndC |
672260 |
2.8.1.7 | C327S/S329C |
inactive |
726296 |
2.8.1.7 | C328A |
IscS mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected |
645632 |
2.8.1.7 | C328S |
inactive |
725215 |
2.8.1.7 | C358A |
CSD mutant, activity towards L-cysteine is almost completely abolished, activity toward L-selenocysteine is much less affected |
645632 |