EC Number |
Protein Variants |
Reference |
---|
2.7.8.27 | H285A |
site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology |
691074 |
2.7.8.27 | H328A |
site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology |
691074 |
2.7.8.27 | H332A |
site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology |
691074 |
2.7.8.27 | more |
construction of an SMS1 interference vector, enzyme silencing by shRNA in Hep-G2 cells leading a downregulation rate of 45% and reduced enzyme activity of 56% |
738307 |
2.7.8.27 | more |
construction of an SMS2 interference vector, enzyme silencing by shRNA in Hep-G2 cells leading to a downregulation rate of 67% and reduced enzyme activity of 63% |
738307 |
2.7.8.27 | more |
downregulation of SMS in Huh7 cells by expression of siRNA, overview |
691046 |
2.7.8.27 | more |
downregulation of SMS1 or SMS2 by siRNA oligonucleotides targeting SMS1 or SMS2 in HeLA cells reduces de novo synthesis of sphingomyelin, overview |
690832 |
2.7.8.27 | more |
endogenous SMS2 expression in HeLa cells is reduced by about 80% after treatment with SMS2 siRNA, but is unaffected after treatment with lamin A or SMS1 siRNAs. Impact of SMS1 and SMS2 depletion on the lipid composition of HeLa cells, overview |
692991 |
2.7.8.27 | more |
generation of SMS2 KO mice |
-, 737776 |
2.7.8.27 | more |
knockout of SMS1 in T-cells by transfection of SMS1-short-interfering RNA into Jurkat T cells, impaired TCR signaling through dysfunction of lipid rafts in SMS1-knockdown T cells, overview. CD3-induced tyrosine phosphorylation and association of linker for activation of T cell with ZAP-70 and Grb2 and phosphorylation of protein kinase C are also severely impaired in Jurkat-SMS1/kd cells |
692624 |