EC Number |
Protein Variants |
Reference |
---|
2.7.7.B22 | A174V |
substitution close to the putative N-terminal DNA-binding domain of TnpA, reduces immunity by approximately sixfold. Transposition activity is comparable to that of wild-type |
762275 |
2.7.7.B22 | C130A/C133A |
mutant losES detectable DNA binding |
762411 |
2.7.7.B22 | C130A/C133A |
mutations in the BED zinc-finger domain, result in an unspecific nuclease activity |
762411 |
2.7.7.B22 | C402A/H405A |
mutant binds DNA normally and is critical for hairpin formation |
762411 |
2.7.7.B22 | C402A/H405A |
mutant completely blocks hairpinning and switching, but still generates nicks in the DNA |
762411 |
2.7.7.B22 | D228N/E648Q |
site-directed mutagenesis |
739693 |
2.7.7.B22 | D310A |
mutation in the catalytic DDE-motif, catalytically inactive |
762411 |
2.7.7.B22 | D377A |
mutation in the catalytic DDE-motif, catalytically inactive |
762411 |
2.7.7.B22 | E279D |
a catalytically inactive transposase mutant |
739149 |
2.7.7.B22 | E54K/L372P |
mutations lead to a hyperactive Tn5 transposase |
762086 |