EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.31 | A445T |
natural variant of TdT, decrease in polymerase ability |
738328 |
| 2.7.7.31 | D179A |
mutation improves templated-polymerase activity |
739723 |
| 2.7.7.31 | D280H |
natural variant of TdT |
738328 |
| 2.7.7.31 | del1-232 |
mutant shows no appreciable activity |
662572 |
| 2.7.7.31 | del1-280 |
mutant shows no appreciable activity |
662572 |
| 2.7.7.31 | del421-501 |
mutant shows no appreciable activity |
662572 |
| 2.7.7.31 | del453-501 |
mutant shows no appreciable activity |
662572 |
| 2.7.7.31 | G257A/V258A/G259A |
mutant shows no appreciable activity |
662572 |
| 2.7.7.31 | K177N/E178G/K271E/F280L/M283K/H405P |
the thermostable mutant TdT3-2 shows a 10 C increased melting temperature as compared to the wild type enzyme. The mutant is capable of incorporating higher amount of ddCTP onto double stranded blunt end DNA |
760281 |
| 2.7.7.31 | L397S |
natural variant of TdT, impaired polymerase activity in vitro and impaired N-additions in an in vivo V(D)J assay |
738328 |
