EC Number   |
Protein Variants |
Reference |
|---|
    2.7.7.18 | A86W/Y118N |
increase in activity, and mutant accepts nicotinamide ribonucleotide as substrate |
-, 760386 |
| 2.7.7.18 | C41A |
active site mutant, increase in Km values |
761694 |
| 2.7.7.18 | C41A/C43A |
active site mutant |
761694 |
| 2.7.7.18 | C43A |
active site mutant, 10fold increase in vmax value |
761694 |
| 2.7.7.18 | D110A |
site-directed mutagenesis replacing the conserved catalytic site, inactive mutant |
739510 |
| 2.7.7.18 | D14A |
site-directed mutagenesis, almost inactive mutant |
-, 738672 |
| 2.7.7.18 | E198P/L217R |
mutation disrupts the dimer interface, leading to a mixture of dimer and monomer in solution |
760983 |
| 2.7.7.18 | H17A |
site-directed mutagenesis, almost inactive mutant |
-, 738672 |
| 2.7.7.18 | H20A |
site-directed mutagenesis, almost inactive mutant |
-, 738672 |
| 2.7.7.18 | K47A |
site-directed mutagenesis, the mutant shows a decrease in Km for ATP and a 6fold increase in Km for nicotinate beta-D-ribonucleotide and a nearly abolished enzyme activity |
-, 738672 |
