EC Number |
Protein Variants |
Reference |
---|
2.7.1.30 | A137S |
affinity for substrates increased 3-4 fold |
641315 |
2.7.1.30 | A344V |
the mutant shows a higher melting temperature than the wild type enzyme |
-, 738722 |
2.7.1.30 | A344V/T386I/F388Y |
the mutant with decreased catalytic efficiency shows a higher melting temperature than the wild type enzyme |
738722 |
2.7.1.30 | A65T |
crystal structure |
641320 |
2.7.1.30 | A65T |
oligomeric interactions are disturbed by the amino acid substitution |
701931 |
2.7.1.30 | C256R |
site-directed mutagenesis, mutation Gyk766AtoG, inactive mutant |
761058 |
2.7.1.30 | C292A |
the mutant shows a lower melting temperature than the wild type enzyme |
-, 738722 |
2.7.1.30 | D20A |
about 95% activity compared to the wild type enzyme |
739102 |
2.7.1.30 | D72V |
the catalytic properties of the mutant differ little from those of the wild type enzyme. The mutant shows 14.76% expression compared to the wild type enzyme |
722712 |
2.7.1.30 | E121C |
mutant protein is labeled with extrinsic fluorophores for FRET |
701931 |