EC Number   |
Protein Variants |
Reference |
|---|
    2.7.1.2 | C166A |
site-directed mutagenesis, mutant shows activity similar to the wild-type enzyme |
661401 |
| 2.7.1.2 | C175A |
site-directed mutagenesis, inactive active site residue mutant |
661401 |
| 2.7.1.2 | C177A |
site-directed mutagenesis, inactive active site residue mutant |
661401 |
| 2.7.1.2 | C182A |
site-directed mutagenesis, inactive active site residue mutant |
661401 |
| 2.7.1.2 | C282A |
site-directed mutagenesis, mutant shows slightly increased enzyme activity compared to the wild-type enzyme, conformational changes different from the wild-type enzyme, overview |
661401 |
| 2.7.1.2 | C321A |
site-directed mutagenesis, mutant shows 5fold increased enzyme activity compared to the wild-type enzyme, conformational changes different from the wild-type enzyme, overview |
661401 |
| 2.7.1.2 | D10K |
site-directed mutagenesis, ATP binding site mutant, inactive mutant |
661401 |
| 2.7.1.2 | more |
construction of an enzyme-deficient glk mutant strain BM5340(DE3), the mutant strain can be complemented by expression of Escherichia coli genes nanK, yajF, and ycfX, which express proteins with 4fold lower glucokinase activity and ambiguous substrate specificities, overview |
661139 |
| 2.7.1.2 | more |
construction of gene sll0593 deletion mutant, does not respond to glucose level in the growth medium, M1 and M2 show reduced activity in glucose growth medium, overview |
662836 |
| 2.7.1.2 | more |
site-specific homologous inactivation of gene glk to construct a mutant strain, activity of the enzyme in isogenic mutants with mutations in the glucose metabolism network, glk mutants exhibit a lower total carbon consumption rate than the parental strain, overview |
-, 680495 |
