EC Number |
Protein Variants |
Reference |
---|
2.7.1.176 | A111C |
deletion of one A in the AAAAAAA tract, which begins at the 78-nt position, results in a frameshift and the stop codon formation after 51 codons, additional mutations are insertion of G after A32 and A111C substitution in one case and A248G substitution in the second one |
717722 |
2.7.1.176 | A248G |
deletion of one A in the AAAAAAA tract, which begins at the 78-nt position, results in a frameshift and the stop codon formation after 51 codons, additional mutations are insertion of G after A32 and A111C substitution in one case and A248G substitution in the second one |
717722 |
2.7.1.176 | D66T |
site-directed mutagenesis |
717773 |
2.7.1.176 | D66T |
site-directed mutagenesis, a nontoxicPezT variant |
717793 |
2.7.1.176 | D66T |
site-directed mutagenesis, a nontoxicPezT variant, no bulge formation or lysis after induction of PezT D66T |
718251 |
2.7.1.176 | D66T/W232Y |
site-directed mutagenesis, a mutated tryptophan-free PezT variant |
717793 |
2.7.1.176 | D67T |
site-directed mutagenesis |
718312 |
2.7.1.176 | K115A |
site-directed mutagenesis, inactive mutant |
761997 |
2.7.1.176 | K45A |
site-directed mutagenesis, the mutation abolishes PezT lethality |
717773 |
2.7.1.176 | K46A |
site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon |
718312 |