EC Number   |
Protein Variants |
Reference |
|---|
    2.6.1.62 | D147N |
site-directed mutagenesis, mutation of an active site residue, inactive mutant |
661088 |
| 2.6.1.62 | F326Y |
mutant improves the recombinant enzyme solubility and stability |
723412 |
| 2.6.1.62 | F326Y |
no major impact on kinetic parameters |
723412 |
| 2.6.1.62 | I793W |
mutant is predicted to obstruct the observed external crevice. Mutant exhibits progress curves with obvious lag phases with extrapolated transient times of 10 to 12 min, as expected for nonchanneling controls |
723412 |
| 2.6.1.62 | I793W |
mutation predicted to obstruct the observed external crevice. In contrast with the wild-type enzyme, mutant exhibits progress curves with an obvious lag phase with extrapolated transient times of 12 min, as expected for nonchanneling controls |
723412 |
| 2.6.1.62 | more |
construction of the gabT NCgl2515-deleted GAD strain SYN203, deletion of the gabT gene encoding GABA-T, and deletion of the additional transaminase gene, NCgl2515, in a gabT-deleted GAD strain |
-, 760076 |
| 2.6.1.62 | R253A |
site-directed mutagenesis, altered kinetics, highly increased Km for S-adenosyl-L-methionine compared to the wild-type enzyme |
661088 |
| 2.6.1.62 | R253K |
site-directed mutagenesis, altered kinetics compared to the wild-type enzyme |
661088 |
| 2.6.1.62 | R253M |
site-directed mutagenesis, altered kinetics compared to the wild-type enzyme |
661088 |
| 2.6.1.62 | R253Q |
site-directed mutagenesis, altered kinetics compared to the wild-type enzyme |
661088 |
