EC Number   |
Protein Variants |
Reference |
|---|
   2.6.1.52 | H41K |
site-directed mutagenesis |
758955 |
| 2.6.1.52 | H41Q |
site-directed mutagenesis |
758955 |
| 2.6.1.52 | H41Y |
site-directed mutagenesis |
758955 |
| 2.6.1.52 | K2G |
site-directed mutagenesis |
759886 |
| 2.6.1.52 | more |
deletion of the 45 N-terminal residues in mutant EhPSAT_DELTA45 results in an inactive protein, the structure shows a dimeric arrangement drastically different from that of the wild-type protein, with the two monomers translated and rotated by almost 180° with respect to each other, causing a rearrangement of the active site to which cofactor PLP is unable to bind. Deletions of first N-terminal 15 (EhPSAT_DELTA15) and four 11th to 14th residues (EhPSAT_DELTA4) yield up to 98% and 90% decrease in activity, respectively. Absence of aldimine linkage between PLP-Lys in the crystal structure of EhPSAT_DELTA4 mutant explains the decrease in activity and describes the importance of these N-terminal residues |
-, 759344 |
| 2.6.1.52 | more |
mutant C2-A' |
759886 |
| 2.6.1.52 | more |
phosphoserine aminotransferase (SerC) from Escherichia coli strain MG1655 is engineered to catalyze the deamination of homoserine to 4-hydroxy-2-oxobutyrate, a key reaction in producing 1,3-propanediol (1,3-PDO) from glucose in a distinct glycerol-independent metabolic pathway. An computation-based rational approach is used to change the substrate specificity of SerC from L-phosphoserine to L-homoserine, molecular dynamics simulations and virtual screening are combined to predict mutation sites. The coexpression of best mutant SerCR42W/R77W with Escherichia coli pyruvate decarboxylase and alcohol dehydrogenase results in production of 3.03 g/l of 1,3-PDO in fed-batch fermentation, which is 13fold higher than in the wild-type strain. Method evaluation, overview |
-, 758955 |
| 2.6.1.52 | more |
PSAT1-silenced lines (ts-psat1.1 and ts-psat1.2) are generated for functional characterization using a microRNA-based approach. Overexpression of the artificial PSAT1-silencing construct in Arabidopsis thaliana leads to a significant reduction of PSAT1 expression, which subsequently results in a strong inhibition of growth. The expression of the PSAT2 gene is unaltered in PSAT1-silenced plants, phenotype, overview |
759278 |
| 2.6.1.52 | more |
PSAT1-silenced lines (ts-psat1.1 and ts-psat1.2) are generated for functional characterization using a microRNA-based approach. The expression of the PSAT2 gene is unaltered in PSAT1-silenced plants, phenotype, overview |
759278 |
| 2.6.1.52 | R42E |
site-directed mutagenesis |
-, 758955 |
