EC Number   |
Protein Variants |
Reference |
|---|
    2.6.1.21 | A77T |
site-directed mutagenesis, the mutation leads to defects in AA metabolism. The mutation A77T leads to a strong decrease in the production of D-Glu and D-Ala with 2-oxoglutarate or pyruvate as substrates, respectively. Instead, the enzymatic defect of AtDAT1(A77T) is quantitatively similar to that of AtDAT1(Ler) |
-, 759259 |
| 2.6.1.21 | A77T/T303S |
naturally occuring mutation in Arabidopsis thaliana accession Ler, sequencing of the genomic locus and the cDNA of AtDAT1 from accession Ler reveals the two missense mutations leading to amino acid exchanges of the protein sequence (A77T and T303S), but solely the A77T amino acid exchange is responsible for the activity loss of AtDAT1(Ler) |
-, 759259 |
| 2.6.1.21 | E32A |
1% of wild-type activity |
636745 |
| 2.6.1.21 | E32D |
65% of wild-type activity |
636745 |
| 2.6.1.21 | E32Q |
10% of wild-type activity |
636745 |
| 2.6.1.21 | H86F |
site-directed mutagenesis, the mutant shows no activity with (R)-PEA and no wild-type DATA activity |
-, 758564 |
| 2.6.1.21 | K33A |
64% of wild-type activity |
636745 |
| 2.6.1.21 | L201A |
- |
636748 |
| 2.6.1.21 | L201A |
2% of wild-type kcat |
-, 636743 |
| 2.6.1.21 | L201W |
0.043% of wild-type kcat |
-, 636743 |
