EC Number |
Protein Variants |
Reference |
---|
2.5.1.B43 | F28I |
naturally occuring mutation, the mutant shows chlorophyll synthase, EC 2.5.1.62, ChlG, activity |
738867 |
2.5.1.B43 | more |
a BchG mutant Rhodobacter sphaeroides BG1 strain is photosynthetically incompetent. Expression of gene chlG from Synechocystis sp. PCC 6803, encoding a chlorophyll synthase I44F mutant, EC 2.5.1.62, rescues the photoheterotrophical growth of the mutant cells. The Synechocystis mutated enzyme ChlGI44F shows phytyl-dependent bacteriohchlorophyll a synthase activity |
738867 |
2.5.1.B43 | more |
improvements to photosynthetic efficiency can be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption via development of organisms that can produce both bacteriochlorophylls and chlorophylls, engineering of the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase results in the production of chlorophyll a. Chlorophyll a can be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, method optimization, overview. Mutant subcloning in Escherichia coli strains JM109 and S17-1 |
737331 |