EC Number |
Protein Variants |
Reference |
---|
2.5.1.87 | E68A |
product distributions is shifted to longer region to give the C65C70 as the main products |
704767 |
2.5.1.87 | K109A |
product distributions is shifted to longer region to give the C65C70 as the main products |
704767 |
2.5.1.87 | L113A |
product distributions is shifted to longer region to give the C65C70 as the main products |
704767 |
2.5.1.87 | more |
construction of the PMET3RER2/rer2D mutant strain JOS18, and of ura3D::imm434/ura3D::imm434 RER2/rer2D::hisG mutant strain JOS14, and of ura3D::imm434/ura3D::imm434 RER2/rer2D::hisG-URA3-hisG mutant strain JOS13 |
-, 738313 |
2.5.1.87 | more |
generation a triple deletion strain, nus1DELTA/rer2DELTA/srt1DELTA, expressing the homomeric cis-PTase from Giardia lamblia (GlcisPT), GlcisPT on a plasmid with a URA3 marker, to support growth, functional complementation by recombinant expression of active human and Schizosaccharomyces pombe enzymes |
-, 738001 |
2.5.1.87 | more |
generation of NgBR knockout mice |
738001 |
2.5.1.87 | more |
generation of RNAi-mediated knockdown of SlCPT3 expression in plants, SlCPT3 gene expression and also polyisoprenoid contents are reduced by approximately 60% and 40%, respectively. Although transgenic plantlets are recovered that exhibit a higher degree of SlCPT3 knockdown, they do snot survive to maturity. Interaction analysis in planta of SlCPT3 and SlCPTBP proteins by introducing C-terminally Myc-tagged SlCPT3 and FLAG-tagged SlCPTBP versions into Nicotiana benthamiana leaves in the endoplasmic reticulum lumeninal side |
-, 739317 |
2.5.1.87 | R290H |
a naturally occuring NgBR R290H mutation leading to congenital disorder of glycosylation. cis-PTase activity and mannose incorporation into proteins is markedly lower in NgBR R290H fibroblasts compared to control, the NgBR R290H mutant is a loss of function mutation that affects cis-PTase function of NgBR without disrupting complex formation with hCIT or Nogo-B. The reduced cis-PTase activity in fibroblasts is manifested as altered dolichol profiles in the urine or serum as assessed by mass spectrometry of all carriers of the R290H mutation, phenotype, overview |
738001 |