EC Number |
Protein Variants |
Reference |
---|
2.5.1.68 | I59A |
change in product chain-length, produces prenyl products with chain length up to C50 |
690753 |
2.5.1.68 | I59F |
inactive mutant enzyme |
690753 |
2.5.1.68 | I59M |
change in product chain-length, produces prenyl products with chain length up to C50 |
690753 |
2.5.1.68 | I59V |
change in product chain-length, produces prenyl products with chain length up to C50 |
690753 |
2.5.1.68 | L84A |
potentially important in limiting chain length. The mutant L84A is not expressed in a soluble form |
-, 705121 |
2.5.1.68 | L84A/L85F |
mutant enzyme has a Km-value for geranyl diphosphate of 0.15 mM compared to 0.038 mM for wild-type Rv108612 |
-, 705121 |
2.5.1.68 | L84A/L85F |
well expressed and active. Mutant protein is capable of synthesizing prenyl diphosphates containing 20 carbon atoms when geranyl diphosphate is used as the allylic substrate and 50 carbon atoms when EE-farnesyl diphosphate is used as the allylic substrate |
-, 705121 |
2.5.1.68 | V107G |
inactive |
-, 705121, 738977 |