EC Number   |
Protein Variants |
Reference |
|---|
   2.5.1.148 | A288F |
significant loss of lycopaoctaene production when compared to wild-type. Increased residue size in the mutant blocks binding of geranylgeranyl diphosphate in the S1 pocket |
749460 |
| 2.5.1.148 | M180L |
mutant retains the ability to produce lycopaoctaene |
749460 |
| 2.5.1.148 | more |
mutating Ser276 and Ala288 residues to those found in human squalene synthase largely converts LOS from lycopaoctaene production to C30 squalene production |
749460 |
| 2.5.1.148 | S276Y |
significant loss of lycopaoctaene production when compared to wild-type. Increased residue size in the mutant blocks binding of geranylgeranyl diphosphate in the S1 pocket |
749460 |
| 2.5.1.148 | S276Y/A288F |
mutant shows a drastic reduction in the ability to produce lycopaoctaene and C35H58 compared to wild-type enzyme |
749460 |
| 2.5.1.148 | T65V |
mutant retains the ability to produce lycopaoctaene |
749460 |
| 2.5.1.148 | T65V/A288F |
when comparing the M7 double mutant to the parent A288F background, T65V/A288F does not show a difference in lycopaoctaene production, but C35H58 production is slightly increased and squalene formation is significantly reduced |
749460 |
| 2.5.1.148 | T65V/M180L |
activity of the mutant enzyme is similar to that of the wild-type enzyme |
749460 |
| 2.5.1.148 | T65V/M180L/A288F |
mutant shows activity similar to that of the M4 parent background |
749460 |
| 2.5.1.148 | T65V/M180L/S276Y A288F |
in comparison to the M9 parent background, the mutant enzyme shows a further reduction in C35H58 production and no significant change in lycopaoctaene and squalene formation |
749460 |
