EC Number   |
Protein Variants |
Reference |
|---|
   2.4.2.36 | D461A |
part of active-site loop 1 (453-463) |
692077 |
| 2.4.2.36 | D463A |
part of active-site loop 1 (453-463) |
692077 |
| 2.4.2.36 | DELTA424-634 |
catalytic domain of cholix has much greater affinity for NAD+ than the full-length enzyme. 15fold higher ADP-ribosyltransferase activity than full-length enzyme |
722762 |
| 2.4.2.36 | E546A |
reduced ADPRT activity compared to wild-type possibly due to leakage of aqueous solvent into binding pocket which prevents ADP-ribose transfer, no impaired NAD+ binding and glycohydrolase activity, Glu546 crucial for ADPRT activity of ExoA but not strictly conserved among toxin family members, part of active-site loop 3 (546-551), upon NAD+-binding Glu546 and Tyr481 (both connected by hydrogen bonds resulting in water molecule exclusion) in hydrogen bonding distance to nucleophilic N3 of diphthamide imidazole possibly increasing its nucleophilic character |
692077 |
| 2.4.2.36 | E546A/R551A |
double alanine mutant, almost complete loss of ADPRT activity not restored by mutations E546D/R551K or E546R/R551E |
692077 |
| 2.4.2.36 | E546D |
lesser ADPRT activity than E546A |
692077 |
| 2.4.2.36 | E546F |
part of active-site loop 3 (546-551) |
692077 |
| 2.4.2.36 | E546H |
see E546A |
692077 |
| 2.4.2.36 | E546N |
partial rescue of ADPRT activity compared to E546A |
692077 |
| 2.4.2.36 | E546Q |
part of active-site loop 3 (546-551) |
692077 |
