EC Number   |
Protein Variants |
Reference |
|---|
    2.4.2.14 | A417W |
moderate resistance to inhibition by ATP or GPT |
657611 |
| 2.4.2.14 | C87A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
759267 |
| 2.4.2.14 | D310V |
almost resistant to inhibition by ATP or GTP, low concentrations of ATP plus GTP activate |
657611 |
| 2.4.2.14 | D432A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
759267 |
| 2.4.2.14 | D433A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
759267 |
| 2.4.2.14 | G331I |
50% of wild-type glutaminase activity, reduced inhibition by GMP, enhanced inhibition by AMP |
489763 |
| 2.4.2.14 | I198V |
by missense mutation A592G, purF(I198V) and variant purF(1-04) in pCA24N by two polymerase error prone PCR strategy (1. mutazyme, 2. Taq DNA polymerase in Thermopol reaction buffer) followed by second directed mutagenesis on purF(1-04) by passaging through Escherichia coli mutator strain XL1-Red leading to variant purF(2-02), mutant I198V and variants 1-04 and 2-02 improved growth of Escherichia coli JMB9 DELTA TrpF (auxotrophic for tryptophan) on medium lacking tryptophan by gained phosphoribosylanthranilate isomerase activity |
693634 |
| 2.4.2.14 | K326Q |
insensitive to inhibition by GMP, reduced synergistic inhibition |
489765 |
| 2.4.2.14 | K326Q |
similar glutaminase and amidotransferase activity as wild-type, not inhibited by GMP |
489763 |
| 2.4.2.14 | K326Q/P410W |
binding of GMP and AMP is abolished resulting in loss of inhibition |
489765 |
