EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.82 | more |
construction of two independent loss-of-function mutants for gene RFS5, i.e. rs 5-1 and rs 5-2. Leaves of mutant plants fail to accumulate any raffinose under diverse abiotic stresses including water-deficit, high salinity, heat shock, and methyl viologen-induced oxidative stress. Correlated to the lack of raffinose under these abiotic stress conditions, both mutant plants lack the typical stress-induced raffinose synthase activity increase observed in the leaves of wild-type plants. The seeds of both mutant plants still contain raffinose Phenotypes, overview |
735861 |
| 2.4.1.82 | more |
generation of two independent maize (Zea mays) zmrafs mutant lines, in which raffinose is completely abolished, the mutants are more sensitive to chilling stress and their net photosynthetic product (total soluble sugars and starch) accumulation is significantly decreased compared with controls after chilling stress. Construction of a mutant of the maize dehydration responsive element (DRE)-binding protein 1A (zmdreb1a), showing significantly decreased ZmRAFS expression and raffinose content compared with the control under chilling stress. Overexpression of maize ZmDREB1A in maize leaf protoplasts increases ZmDREB1A amounts, which consequently upregulate the expression of maize ZmRAFS and the Renilla luciferase (Rluc), that is controlled by the ZmRAFS promoter. Deletion of the single dehydration-responsive element (DRE) in the ZmRAFS promoter abolishes ZmDREB1A's influence on Rluc expression, while addition of three copies of the DRE in the ZmRAFS promoter dramatically increases Rluc expression when ZmDREB1A is simultaneously overexpressed |
-, 759940 |
| 2.4.1.82 | more |
isolation of a T-DNA insertional mutant in the AtRS4 gene, only semi-quantitative PCR from wild-type siliques shows a specific transcriptional AtRS4PCR product. Metabolite measurements in seeds of DELTAAtRS4 mutant plants reveal a total loss of stachyose in DELTAAtRS4 mutant seeds |
-, 736162 |
| 2.4.1.82 | S150F |
line 165, mutation induced by mutagen ethyl-methanesulfonate, seed phenotype does not differ from wild-type |
702924 |
| 2.4.1.82 | T107I |
line 397, mutation induced by mutagen ethyl-methanesulfonate, seed sucrose is increased by 28%, raffinose is reduced to 37% and stachyose is reduced to approximately 24% compared to wild-type |
702924 |
