EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.257 | D203A |
mutation has no influence on Alg2 function |
709008 |
| 2.4.1.257 | D248A |
mutation has no influence on Alg2 function |
709008 |
| 2.4.1.257 | E264A |
mutation has no influence on Alg2 function |
709008 |
| 2.4.1.257 | E335A |
mutant has some residual activity |
709008 |
| 2.4.1.257 | E335A |
significant lower level of product formation |
707472 |
| 2.4.1.257 | E335A |
site-directed mutagenesis, Trx-scAlg2E335A produces only no final product and only 32% of intermediate Man2Gn2 compared to wild-type enzyme |
759165 |
| 2.4.1.257 | E335A/E343A |
significant lower level of product formation, identical to that of the E335A mutant |
707472 |
| 2.4.1.257 | E343A |
inactive mutant enzyme |
709008 |
| 2.4.1.257 | E343A |
no activity |
707472 |
| 2.4.1.257 | E343A |
site-directed mutagenesis, inactive mutant |
759165 |
